Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Pterostilbene prevents intestinal ischemia reperfusion injury in Wistar rats via modulation of antioxidant defense and inflammation

Yang Sun¹, Yang Xu², Guo-Nian Wang²

¹Department of Anesthesiology, Heilongjiang Province Hospital; ²Department of Anesthesiology, The Third Affiliated Hospital of Harbin Medical University, Heilongjiang 150000, China.

For correspondence:- Guo-Nian Wang Email: guonianwang0188@gmail.com Tel:+8645186298811

Received: 19 February 2015 Accepted: 29 June 2015 Published: 30 August 2015

Citation: Sun Y, Xu Y, Wang G. Pterostilbene prevents intestinal ischemia reperfusion injury in Wistar rats via modulation of antioxidant defense and inflammation. Trop J Pharm Res 2015; 14(8):1383-1391 doi: 10.4314/tjpr.v14i8.9

© 2015 The authors.
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Abstract

Purpose: To evaluate the protective mechanisms afforded by pterostilbene against intestinal ischemia/reperfusion ( II/R ) injury in Wistar rats.

Methods: Male Wistar rats were divided into 4 groups as follows: Control group; intestinal ischemia/reperfusion (II/R) group; pterostilbene only group (20 mg/kg) of body weight and pterostilbene followed by intestinal ischemia/reperfusion (II/R) treated group. The study evaluated oxidative stress markers, including reactive oxygen species (ROS) and lipid peroxide levels, protein carbonyl content, antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione content) and membrane-bound ATPase activity. The levels of pro-inflammatory mediators, including nuclear factor- kappa B (NF-x581;B), cyclooxygenase-2 (COX-2) and inflammatory cytokines (TNF-α and IL-1β), were also evaluated.

Results: The results showed that pterostilbene (20 mg/kg) followed by intestinal ischemia/reperfusion (II/R) significantly lowered the level of lipid peroxidation (41.33 %), protein carbonyl content (PCC, 44.18 %) and ROS (29.14 %) (p < 0.001) but significantly restored membrane-bound ATPase activities (Ca²⁺ATPase, 30.76 %; Na⁺/K⁺ATPase, 21.42 %; Mg²⁺ATPase, 30.06 %) (p < 0.003), compared with rats induced with II/R. Furthermore, pterostilbene significantly down-regulated NF-x581;B and COX-2 expressions (30 %, p < 0.05) compared to rats with II/R injury.

Conclusion: The study reveals that pterostilbene offers significant protective activity in rats owing to its antioxidant and anti-inflammatory properties.

Keywords: Pterostilbene, Intestinal reperfusion, Ischemia, Inflammation, Antioxidant, Oxidative stress